Research Article

Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization

Figure 3

Chromatographic separation of trypsin solutions obtained by ultrafast high performance liquid chromatography (UF-HPLC) (a). Trypsin solution concentrations ranged from 52 μM (100%) to 1.3 μM (2.5%), as shown in the color scale. Acetonitrile concentration gradient linearly varied from 5 to 65% in 15 min. Standard curve obtained by integrating the area under the peaks relating to the trypsin corresponding fraction (b).