Research Article
Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization
Table 1
Experimental, coded, and uncoded planning matrix for the central compound design (CCD) for pH and immobilization time.
| | Experiment number | pH | Time (min) |
| | 1 | 6.0 (−1) | 30 (−1) | | 2 | 6.0 (−1) | 90 (+1) | | 3 | 8.6 (+1) | 30 (−1) | | 4 | 8.6 (+1) | 90 (+1) | | 5 | 5.8 (−1.26) | 60 (0) | | 6 | 9.24 (+1.26) | 60 (0) | | 7 | 7.6 (0) | 21.98 (−1.26) | | 8 | 7.6 (0) | 98.01 (+1.26) | | 9 (C) | 7.6 (0) | 60 (0) | | 10 (C) | 7.6 (0) | 60 (0) | | 11 (C) | 7.6 (0) | 60 (0) | | 12 (C) | 7.6 (0) | 60 (0) | | 13 (C) | 7.6 (0) | 60 (0) |
|
|
