Preparation and Characterisation of Nobiletin-Loaded Nanostructured Lipid Carriers

Table 2

Observed response in Box-Behnken design for preparation and optimization of NOB-NLC formulations with predicted values generated by Design Expert software.

Formulations	Independent variables			Dependent variables
				Observed value		Predicted value
				(1)	1.00	0.50	1.00			157.26	71.42
(2)	2.50	0.50	1.00			172.72	83.38
(3)	1.00	2.00	1.00			174.92	87.24
(4)	2.50	2.00	1.00			121.38	68.17
(5)	1.00	1.25	0.33			172.34	81.30
(6)	2.50	1.25	0.33			177.96	75.77
(7)	1.00	1.25	1.67			149.65	59.94
(8)	2.50	1.25	1.67			105.95	58.36
(9)	1.75	0.50	0.33			190.08	82.62
(10)	1.75	2.00	0.33			175.67	83.38
(11)	1.75	0.50	1.67			145.17	63.69
(12)	1.75	2.00	1.67			125.89	63.54
(13)	1.75	1.25	1.00			102.06	83.67
(14)	1.75	1.25	1.00			102.06	83.67
(15)	1.75	1.25	1.00			102.06	83.67
(16)	1.75	1.25	1.00			102.06	83.67
(17)	1.75	1.25	1.00			102.06	83.67

= emulsifier-to-lipid ratio, = lecithin-to-Poloxamer 188 ratio, = liquid lipid-to-solid liquid ratio, = mean particle size (nm), and = encapsulation efficiency (%).