Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus

Figure 4

(a) Cyclic voltammograms (CV) and (b) Nyquist plots of (i) bare SPCE strip; (ii) CNPs modified SPCE strip; (iii) JEV antibody immobilized CNPs on SPE strip; (iv) BSA blocked/JEV antibody immobilized CNPs on SPCE strip; (v) BSA blocked/JEV antibody immobilized CNPs on SPCE strip in the presence of JEV; (vi) JEV positive human serum; (vii) Dengue antigen. Both CV and EIS were conducted in PBS solution (pH 7.4) containing 0.1 M KCl and 15 mM of K₃[Fe(CN)₆]/K₄[Fe(CN)₆] at a scan rate of 50 mVs⁻¹ and frequency range of 0.01 Hz to 100.0 kHz, respectively. [Inset of 4B shows electron transfer resistance, , for various Nyquist plots (i–vii) and the Randles equivalent circuit.]

(a)

(b)