Polymalic Acid Tritryptophan Copolymer Interacts with Lipid Membrane Resulting in Membrane Solubilization

Figure 3

P/WWW liposome particles formed by mixing and physical agitation were analyzed by size-exclusion HPLC (SE-HPLC). (a) SE-HPLC elution profiles of solutions containing liposome, PMLA copolymers P/WWW, or P/LOEt (panel (a), (D)–(F)), and mixtures of liposome and copolymers at different lipid to copolymers molar ratios for liposome/P/WWW (panel (a), (A)–(C)) and liposome/P/LOEt (panel (a), (G)). (b) The UV-vis spectrum of the peak in panel (a) was analyzed by built-in DAD module (diode array detector). Upper spectrum represented the peak in curve (A) (panel (a)) indicated by the characteristic 280 nm absorbance of tryptophan in liposome/P/WWW complex and the lower spectrum represented the peak of liposome in curve (D). (c) The hydrodynamic diameters of P/WWW (green), liposome (blue), and mixtures P/WWW with liposome (red) were , , and  nm by dynamic light scattering. (d) The morphology of the P/WWW liposome particle (same composition as panel (a) liposome/copolymer weight ratio 2 : 1, curve (A)) was analyzed by transmission electron microscopy (TEM). The particle appears to be round in shape and of varying diameter suggesting that the particles derived from liposome/P/WWW were polydisperse with an average size close to 10 nm.

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