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Journal of Nanomaterials
Volume 2017, Article ID 6934757, 9 pages
https://doi.org/10.1155/2017/6934757
Research Article

Studies of the Influence of Gold Nanoparticles on Characteristics of Mesenchymal Stem Cells

1Department of Cryopathophysiology and Immunology, Institute for Problems of Cryobiology and Cryomedicine, The National Academy of Sciences of Ukraine, Pereyaslavskaya Str. 23, Kharkov 61015, Ukraine
2Department of Reproductive Systems, Institute for Problems of Cryobiology and Cryomedicine, The National Academy of Sciences of Ukraine, Pereyaslavskaya Str. 23, Kharkov 61015, Ukraine
3International Surface Enhanced Spectroscopy (SES) Laboratory, Institute of Physics, The National Academy of Sciences of Ukraine, 46 Nauki Ave., Kyiv 03028, Ukraine

Correspondence should be addressed to Nataliia Volkova; au.xednay@6002ilatanavoklov

Received 27 July 2017; Accepted 20 September 2017; Published 24 October 2017

Academic Editor: Faheem Ahmed

Copyright © 2017 Nataliia Volkova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the present study is to determine what effect the different concentrations of 15 nm gold nanoparticles (AuNPs) will have on the immunophenotype, synthesis collagen type I, ability to direct differentiation and spectroscopic characteristics of bone marrow mesenchymal stem cells (MSCs). The AuNPs in concentrations of 1.5–9 μg/ml did not lead to changes in the level of expression of CD 45, CD 90, and CD 73. It should be noted that AuNPs in concentrations of 6 and 9 μg/ml led to a decrease in CD 44 cells by 6% and 9%, respectively. The content of CD 105 cells was reduced by 5% when AuNPs were applied at a concentration of 9 μg/ml. It was found that AuNPs in concentrations of 1.5–6 μg/ml are safe for MSCs, while the increase up to 9 μg/ml has a toxic effect, manifested by the reduction of synthesis collagen type I and ability of adipogenic differentiation. IR spectroscopy data have shown that the AuNPs at concentrations of 9 μg/ml under conditions of adipogenic differentiation to MSCs lead to the destruction processes in the cells. The obtained results are related to the field of applied nanotechnology, which extends to regenerative medicine, especially in development of bioimplantology.