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Journal of Nanomaterials
Volume 2017, Article ID 8542806, 12 pages
Research Article

Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements

1School of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai 200237, China
2China Pharmaceutical University, Nanjing 211198, China
3Health School Attached to Shanghai University of Medicine & Health Sciences, Shanghai 200237, China
4State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
5Center for Bioinformatics and Computational Biology and the Institute of Biomedical Sciences, School of Life Science, East China Normal University, Shanghai 200241, China
6School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
7Institute of Nano Biomedicine and Engineering, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

Correspondence should be addressed to Zhongjian Fang; moc.anis@pivylihs

Received 28 October 2016; Revised 16 January 2017; Accepted 23 January 2017; Published 23 February 2017

Academic Editor: Jian Zhong

Copyright © 2017 Zhongjian Fang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The past two decades have witnessed the great growth of the development of novel drug carriers. However, the releasing dynamics of drug from drug carriers in vivo and the interactions between cells and drug carriers remain unclear. In this paper, liposomes were prepared to encapsulate D-luciferin, which was the substrate of luciferase and served as a model drug. Based on the theoretical calculation of active loading, methods of preparation for liposomes were optimized. Only when D-luciferin was released from liposomes or taken in by the cells could bioluminescence be produced under the catalysis of luciferase. Models of multicellular tumor spheroid (MCTS) were built with 4T1-luc cells that expressed luciferase stably. The kinetic processes of uptake and distribution of free drugs and liposomal drugs were determined with models of cell suspension, monolayer cells, MCTS, and tumor-bearing nude mice. The technology platform has been demonstrated to be effective for the study of the distribution and kinetic profiles of various liposomes as drug delivery systems.