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Journal of Nanomaterials
Volume 2017, Article ID 8734758, 19 pages
Research Article

Phytosynthesis of Silver and Gold Nanoparticles Using the Hot Water Extract of Mixed Woodchip Powder and Their Antibacterial Efficacy

1Department of Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea
2School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea
3Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, Republic of Korea
4Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea
5Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 61186, Republic of Korea

Correspondence should be addressed to Suk Bai;

Received 6 April 2017; Accepted 28 June 2017; Published 11 September 2017

Academic Editor: Jorge Pérez-Juste

Copyright © 2017 Min-Ho Joe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study investigates the phytosynthesis, characterization, and antibacterial efficacy of silver and gold nanoparticles (NPs) produced using the hot water extract of mixed woodchip powder. The woodchip extract (WCE) was successfully used as both a reducing and stabilizing agent for the phytosynthesis of both crystalline metal NPs. The effects of different physicochemical factors affecting the formation of the metal NPs including reaction pH, concentration of the precursor metal salts, amount of WCE, and external energy input were evaluated. The characterization of the metal NPs was performed by transmission electron microscopy, selected area electron diffraction (SAED), energy dispersive X-ray (EDX) spectroscopy, and X-ray diffraction (XRD) pattern analysis. In addition, the antibacterial efficacy of the phytosynthesized NPs was measured. The AgNPs showed clear antibacterial activity against four representative bacterial strains. However, the AuNPs did not exhibit bactericidal activity, probably due to their surface modifications and relatively large size. These results suggest that the phytosynthesis of the metal NPs using WCE is highly efficient, and its convenience makes it suitable for use in large-scale production.