Validation of a Rapid Visual Screening of Campylobacter jejuni in Chicken Using Antibody-Conjugated Fluorescent Dye-Doped Silica Nanoparticle Reporters

Table 2

Calculations of AC, SP, SE, PPV, and NPV values of the proposed assay for detection of C. jejuni in various analysed samples against the direct culture with duplex PCR assay as a reference method.

Analyzed samples	PA	PD	ND	NA	N+ (PA + ND)	N− (NA + PD)	Total	AC (%)	SP (%)	SE (%)	PPV (%)	NPV (%)
Carcass rinses	26	0	4	20	30	20	50	92.00	100	86.67	100	83.33
Rectal swabs	58	0	1	1	59	1	60	98.33	100	98.30	100	50.00
Viscera contents	27	0	1	2	28	2	30	96.67	100	96.42	100	66.67

Note: PA: positive accordance; PD: positive deviations; NA: negative accordance; ND: negative deviations; N: number of analyzed samples; N+: total of positive samples compared with a reference method; N−: total of negative samples compared with a reference method; AC: relative accuracy; SP: relative specificity; SE: relative sensitivity; PPV: positive predictive value; and NPV: negative predictive value.