Topographical Guidance of PSC-Derived Cortical Neurons

Figure 1

Cortical neuron differentiation of human PSCs. (a) Schematic overview of the adapted cortical differentiation protocol by Shi et al. [3]. DIV40 purified cortical neuronal progenitor cells (NPC) were plated on microtopographical substrates. (b) Transcript levels of the pluripotency marker (OCT4), the neural progenitor marker (PAX6), and lower layer cortical neuron markers (TBR1, CTIP2) were assessed at specific time points throughout differentiation for the following PSC lines: the H9 hESC line and the hiPSC lines (0028, CRL-2522, BJ1, and SBAD2). Gene expression was normalized to the expression of the housekeeping gene GAPDH ( differentiations per cell line).

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