Topographical Guidance of PSC-Derived Cortical Neurons

Figure 6

Cortical neuron differentiation of human PSC on microtopography substrates: (a) quantification of total cell numbers, (b) mean neurite outgrowth per cell, and subpopulations of (c) neuronal progenitors (NESTIN positive cells) and (d) neurons (TUJ1 positive cells). Averaged data of cortical differentiations of the SBAD2, 0028, ChiPSC6b, and BJ1 iPSC lines. (e) Quantification of cell number, average neurite outgrowth, and NESTIN expression. Averaged data for each individual PSC line is plotted on top of the combined data (bar chart). Within each time point, we compared flat, groove, and pillar substrates.

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