Effects of Titanium Micro-Nanopermeable Structures on Osteogenic Differentiation
Activation of MAPK signaling pathway of osteoblasts after incubation on Ti materials with different surface modification for 1 d and 4 d. (a) Western blotting analysis of the p38 phosphorylation on Ti materials with different surface modification. Increased p38 phosphorylation with the incubation time and significantly increased p38 phosphorylation could be found on Ti with SLA + HT treatment compared to control and SLA treatment. (b) Quantification of the p38 phosphorylation on day 4. (c) Western blotting of ERK phosphorylation on Ti materials with different surface modification. No ERK phosphorylation was detected from osteoblasts grown on Ti materials with different treatment. Same amount of protein was also extracted from fibroblasts as positive controls. Control: polished Ti; SLA: 400 μm SiO2 sandblast + acid etching; SLA + HT: 400 μm SiO2 sandblast + acid etching + 4 mol/L NaOH hydrothermal for 4 h. .