Journal of Nanomaterials / 2018 / Article / Fig 2

Research Article

Porous Maltodextrin-Based Nanoparticles: A Safe Delivery System for Nasal Vaccines

Figure 2

Evaluation of the cytotoxicity of NPL. The mitochondrial activity (referred to as viability (a, c)) and the membrane integrity (referred to as mortality (b, d)) in H292 airway (a, b) and Caco2 intestinal (c, d) epithelial cells were, respectively, determined by the MTT and the LDH assays. Cells were seeded in a 96-well plate and treated with NPL from 0 (negative control) to 150 μg/cm2. The cells were analyzed with the MTT assay while the supernatants of treated cells were collected for the LDH assay. Positive controls (+) correspond to cells treated with either 4% (v/v) paraformaldehyde to induce the minimal MTT value or with 10% (v/v) Triton X-100 to induce the maximal LDH release, and 100% was set over the negative control. Data represent the mean ± SD of the percentage of viability or mortality. No significant difference was observed between 0 (untreated cells) and the NPL-treated cells.

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