Porous Maltodextrin-Based Nanoparticles: A Safe Delivery System for Nasal Vaccines
Induction of reactive oxygen species (ROS) by NPL. H292 airway (a) or Caco2 intestinal (b) epithelial cells were seeded in a 96-well plate and probed with H2DCF-DA to detect ROS. Cells were then treated with vehicle (control) or with 150 μg/cm2 of NPL and immediately recorded for ROS induction using the Fluoroskan Ascent (Thermo Scientific, France) at the wavelengths (excitation/emission) 488 nm/527 nm. Data represent the mean fluorescence intensity ± SD normalized over the initial time arbitrarily set to 1. No significant difference was observed over the negative control. Not shown: positive control (100 μM TBHP) vs NPL treatment.
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