TY - JOUR A2 - Morais, Paulo Cesar AU - Lin, Haitao AU - Huang, Jun AU - Ding, Liyun PY - 2019 DA - 2019/10/17 TI - Preparation of Carbon Dots with High-Fluorescence Quantum Yield and Their Application in Dopamine Fluorescence Probe and Cellular Imaging SP - 5037243 VL - 2019 AB - Carbon dots represent a kind of fluorescent nanomaterial and have broad application prospects in the field of biosensing and optoelectronics. Here, we explored carbon dots with a high-fluorescence quantum yield rate synthesized from L-cysteine and citric acid by the microwave-assisted method. The characteristics of the carbon dots were studied using a transmission electron microscope, an X-ray diffractometer, X-ray photoelectron spectra, a UV-Vis absorption spectrometer, a FTIR spectrometer, and a fluorescence spectrophotometer. The obtained carbon dots exhibited a high-fluorescence quantum yield (up to 85%), which is due to the combination of amidogens and sulfydryl with carbon dots, and hence bring the improved fluorescence property. We used carbon dots for in vitro imaging of CRL-5822 cells and human umbilical vein endothelial cells, which showed the low inhibitory rate (0.8%) of cells for 48 h with good biocompatibility demonstrated by the cell viability assay. The image of cells can be observed clearly under UV light. The Stern-Volmer equation was introduced to describe the quenching effect between the fluorescence intensity of carbon dots and the concentration of aqueous dopamine (DA). SN - 1687-4110 UR - https://doi.org/10.1155/2019/5037243 DO - 10.1155/2019/5037243 JF - Journal of Nanomaterials PB - Hindawi KW - ER -