Biological Characteristics and Carrier Functions of Pegylated Manganese Zinc Ferrite Nanoparticles

Figure 5

Construction and identification of CD44-shRNA eukaryotic expression plasmid. (a) Eletrophoretograms of CD44-shRNA identified by PCR. Lanes 1 and 3: negative clone; Lanes 2 and 4–8: positive clone; Lane M: DNA marker (the standard bands were 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp in succession from the up to the bottom. (b) CD44-shRNA sequencing comparison results. The sequencing comparison confirmed there are correct clones indicating successful construction of the CD44-v6-shRNA plasmid.

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