Nanoencapsulation of Curcumin and Its Protective Effects against CCl₄-Induced Hepatotoxicity in Mice

Figure 4

Photomicrographs of liver sections stained by HE from (a) normal control mice showing that the hepatocytes are polyhedral in shape, with central rounded vesicular nuclei and a acidophilic granular cytoplasm (×100); (b) CCl₄ model control mice showing disorganized hepatic architecture with multiple areas of necrosis with apoptotic cell and apoptotic body distinguished by dense eosinophilic cytoplasm and pyknotic nucleus and mononuclear cellular infiltration around the portal tract with branching of bile ductules and proliferation (×100); (c) free curcumin treatment mice showing mild mononuclear cellular infiltrate mildly dilated and congested portal vein and normal bile duct (×100); (d) curcumin-loaded nanoliposome treatment mice showing organized hepatic architecture with vesiculated nuclei and preserved central vein and a few numbers of hepatocytes show deeply stained acidophilic cytoplasm and dark nuclei (×100); (e) silybin treatment mice showing more or less normal portal vein, preserved bile ducts, and a few numbers of hepatocytes show deeply stained acidophilic cytoplasm and dark nuclei (×100).

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