Characterization and Evaluation of HGF-Loaded PLGA Nanoparticles in a CCl4-Induced Acute Liver Injury Mouse Model
Morphological and histopathological examination of the liver tissue to investigate the influence of HGF-loaded PLGA nanoparticles on acute liver injury induced by CCl4 in experimental mice. (a) Representative images of the livers of the investigated groups, which included the normal, untreated, NPs, HGF 60 ng, HGF 80 ng, HGF-2 μg-NPs, and HGF-4 μg-NPs groups. The HGF solution groups were injected with a dose of 60 ng HGF or 120 ng HGF through the tail vein to provide the mice an initial concentration of 40 ng HGF per milliliter of blood (40 ng/ml HGF) or 80 ng HGF per milliliter of blood (80 ng/ml HGF), respectively. Approximately 10 mg HGF-2 μg-NPs or HGF-4 μg-NPs was administered into the blood, and they could release 60 or 120 ng HGF, respectively, until the mice were sacrificed. The ruler units are in mm. (b) The liver index of the investigated groups. The liver index was calculated by dividing the liver weight by the mouse weight. versus the untreated group. (c) Histological analysis of liver sections by hematoxylin and eosin staining. Necrotic areas are encircled with black lines. The pathologic changes of livers from all groups were similar, including necrosis, fatty changes in hepatocytes, and aggregates of inflammatory cells. (d) The injury rate of the tested groups. The injury rate was calculated as the necrotic area divided by the total area under which five fields of the view were analyzed for each group. versus the untreated group. (e) HGF levels in the blood. (f) HGF levels in the liver homogenate. versus the normal group, & versus the HGF 60 ng group, and # versus the HGF 120 ng group.
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