A Review of the Material Characteristics, Antifreeze Mechanisms, and Applications of Cryoprotectants (CPAs)

Table 2

Cryopreservation of various cells using COOH-PLL.

Types of cells	Optimum concentration (%wt)	Ratio of carboxylation	Viability (%)	Viability of control group (10%DMSO) (%)	Combination	Reference
L929 cell	7.5	0.65	>95	~80	None	[19]
Bovine fibroblast cells	5	0.65	96.5	~85	None	[38]
Bovine cumulus cells	20	0.65	95.3	~90	None	[38]
hBMSC	7.5	0.65	>95	~85	None	[39]
Islet cells	20	None	74	No report	30%wt ethylene glycol	[40]
Bull semen	0.75 ()	None	~80	No report	glycerol	[41]
iPS cell	10	0.65	61.6	No report	0.75 M sucrose, 6.5 M ethylene glycol	[42]