Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat
Table 4
Effect of dietary ALA content on the expression of SREPB1c, SCD-1, FAS, G3PDH, and leptin mRNA in omental and retroperitoneal adipose tissue.
0.095%en ALA
0.19%en ALA
0.38%en ALA
0.76%en ALA
1.00% en ALA
2.05%en ALA
4.1%en ALA
6.3% en ALA
Omental Adipose Depot
SREBP1c mRNA(1000)
0.88 ± 0.0
0.74 ± 0.0
1.0 ± 0.
0.95 ± 0.0
0.75 ± 0.
0.60 ± 0.0
0.40 ± 0.0
0.34 ± 0.0
SCD-1 mRNA
0.72 ± 0.0
0.43 ± 0.
1.22 ± 0.
1.42 ± 0.
0.67 ± 0.
0.80 ± 0.
0.31 ± 0.
0.43 ± 0
FAS mRNA
0.11 ± 0.0
0.06 ± 0.0
0.17 ± 0.0
0.17 ± 0.00
0.04 ± 0.0
0.05 ± 0.0
0.03 ±0.00
0.06 ± 0.0
G3PDH mRNA
0.06 ± 0.00
0.05 ± 0.0
0.14 ± 0.0
0.25 ± 0.0
0.10 ± 0.0
0.10 ± 0.0
0.04 ± 0.00
0.05 ± 0.0
Leptin mRNA
0.01 ± 0.002
0.01 ± 0.004
0.02 ± 0.006
0.03 ± 0.005
0.02 ± 0.006
0.02 ± 0.006
0.01 ± 0.005
0.009 ± 0.002
PPAR mRNA
0.029 ± 0.003
0.021 ± 0.003
0.020 ± 0.004
0.030 ± 0.004
0.017 ± 0.009
0.018 ± 0.003
0.018 ± 0.005
0.016 ± 0.007
LPL mRNA
1.30 ± 0.26
1.30 ± 0.23
1.97 ± 0.44
1.73 ± 0.16
2.09 ± 0.62
1.47 ± 0.37
0.97 ± 0.36
0.89 ± 0.08
Adiponectin mRNA
0.74 ± 0.09
0.89 ± 0.15
1.57 ± 0.03
1.62 ± 0.08
3.20 ± 0.79
1.22 ± 0.34
1.56 ± 0.57
1.13 ± 0.44
Retroperitonl Adipose Depot
SREBP1c mRNA (104)
0.25 ± 0.0
0.27 ± 0.0
0.39 ± 0.09 c
0.30 ± 0.0
ND
0.21 ± 0.0
0.16±0.0
0.11 ± 0.0
SCD-1 mRNA
1.69 ± 0.41
1.24 ± 0.03
1.45 ± 0.34
1.60 ± 0.20
ND
1.92 ± 0.19
1.01 ± 0.24
0.92 ± 0.11
FAS mRNA
0.11 ± 0.0
0.08±0.00
0.09 ± 0.0
0.11 ± 0.0
ND
0.11 ±0.0
0.03 ± 0.00
0.04 ± 0.00
G3PDH mRNA
0.30 ± 0.0
0.33 ± 0.0
0.29 ± 0.0
0.40 ±0.0
ND
0.41 ± 0.0
0.23 ± 0.0
0.23 ± 0.0
Leptin mRNA
0.04 ± 0.009
0.05 ± 0.008
0.04 ± 0.009
0.06 ± 0.01
ND
0.05 ± 0.006
0.04 ± 0.005
0.03 ± 0.008
Expression of all genes was normalised to the expression of β-actin. Different superscripts denote values which are significantly different as determined by a Duncan’s post hoc analysis (); ND = no data.
