Research Article

Hepatic Gene Expression Profiles Are Altered by Dietary Unsalted Korean Fermented Soybean (Chongkukjang) Consumption in Mice with Diet-Induced Obesity

Table 5

Comparison between cDNA microarray analysis and real-time RT-PCR.

Genbank No.GenecDNA microarrayReal time RT-PCR
HDconHDCHDconHDC

BG085346Acetyl-Coenzyme A acyltransferase 2 (Acaa2)−4.18(1)0.28(2)0.94
AA277495Carnitine palmitoyltransferase 1a, liver (Cpt1a)ND(3)ND1.733.41
BF45709024-dehydrocholesterol reductase (Dhcr24)2.11.450.78
BG063933Dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (Dpagt1)2.33.021.91
AA023077Hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1)−8.950.231.14
AI835105Monoglyceride lipase (Mgll)−8.990.290.62
W82212Phytanoyl-CoA hydroxylase (Phyh)−5.780.371.21
AI893897Paraoxonase 1 (Pon1)2.01.680.69
AA259329Protein kinase, AMP-activated, gamma 1 noncatalytic subunit (Prkag1)2.22.201.41
AA108401Solute carrier family 27 (fatty acid transporter), member 2 (Slc27a2)−4.060.250.86
AA254935Solute carrier family 27 (fatty acid transporter), member 5 (Slc27a5)−11.730.250.83

(1)The symbol double-headed arrow () denotes that there is no significant difference when gene expression in HDcon or HDC groups was compared with the NDcon group. (2)Data are expressed as fold changes, normalized to β-actin mRNA expression, where the values for the NDcon mice were set at 1.00. The analyses were performed in duplicate. (3)ND, no detected.