Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions

Table 2

Data of the four parameters of the 𝛾-H2AX foci analysis in human lymphocytes and their test-retest reliability (bias: mean difference between the two measurements; LoA: 95% limits of agreement; CV: coefficient of variation).

𝛾-H2AX foci parameter	M1; (mean ± SD)	M2; (mean ± SD)	Bias	LoA; (lower/upper)	CV (%)	𝑃 value
Number of 𝛾-H2AX foci per 100 lymphocytes (𝑁)	7.6 ± 4.4	8.4 ± 4.6	0.75	−15.2/13.7	98.5	0.792
Number of 𝛾-H2AX foci per affected cell (𝑁)	1.2 ± 0.2	1.3 ± 0.1	−0.02	−0.36/0.33	11.3	0.800
Percentage of affected cells (%)	5.9 ± 2.6	6.9 ± 4.2	−1.0	−11.9/9.9	88.9	0.579
Diameter of 𝛾-H2AX foci (𝜇m)	0.63 ± 0.07	0.66 ± 0.06	−0.04	−0.16/0.09	8.0	0.334