Vitamin D3 Suppresses Class II Invariant Chain Peptide Expression on Activated B-Lymphocytes: A Plausible Mechanism for Downregulation of Acute Inflammatory Conditions

Figure 2

Bar graph showing the effects of 1 ng/mL of various vitamin D3 compounds on the distribution of TLR-activated CLIP⁺ and B220 subsets of B cells. This figure demonstrates the changes in MFI of CLIP and CLIP⁺ B220 subsets of B cells 15 hrs after administration of different compounds of vitamin D3 versus no treatment or DMSO (control). These results suggest that the active formulation of vitamin D3 may lead to a statistically significant reduction in CLIP expression on polyclonally activated splenic B cells. Administration of the human calcitriol of vitamin D3 () resulted in the greatest overall reduction in the MFI (482.3 versus a baseline of 701.1) for CLIP expression on stimulated B cells. Administration of the active form of vitamin D3 again resulted in statistically significant reduction in CLIP expression on CpG-activated B cells.