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Journal of Nutrition and Metabolism
Volume 2016, Article ID 5905891, 11 pages
Research Article

Effect of Treatment with Salsalate, Menhaden Oil, Combination of Salsalate and Menhaden Oil, or Resolvin D1 of C57Bl/6J Type 1 Diabetic Mouse on Neuropathic Endpoints

1Department of Veterans Affairs Iowa City VA Health Care System, Iowa City, IA 52246, USA
2Veterans Affairs Center for the Prevention and Treatment of Visual Loss, Iowa City, IA 52246, USA
3Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
4Department of Ophthalmology and Visual Science, University of Iowa, Iowa City, IA 52242, USA
5Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa City, IA 52242, USA

Received 6 July 2016; Revised 2 September 2016; Accepted 4 September 2016

Academic Editor: Stan Kubow

Copyright © 2016 Matthew S. Yorek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, menhaden oil, the combination of salsalate and menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad. Results. Diabetic animals failed to gain weight and had elevated blood glucose levels. Diabetic mice had slowed nerve conduction velocity, reduced innervation of the foot pad and cornea subepithelial and epithelial layers, and reduced thermal sensitivity. Monotherapy treatment with salsalate, menhaden oil, and resolvin D1 reduced the pathological signs of diabetic neuropathy. The combination of salsalate and menhaden oil also reduced signs of pathology and generated elevated plasma levels of resolvin D1 compared to other groups. Conclusions. Additional studies are needed to determine whether the combination of salsalate and menhaden oil may be more efficacious than monotherapy alone for the treatment of diabetic peripheral neuropathy.