Downregulation of PGC-1α Prevents the Beneficial Effect of EET-Heme Oxygenase-1 on Mitochondrial Integrity and Associated Metabolic Function in Obese Mice

Figure 1

Effect of EET-A treatment on WT and PGC-1α-deficient 3T3-L1 adipocytes cells on gene expression related to mitochondrial biogenesis and dynamics. mRNA expression of PGC-1α (a), HO-1 (b) in WT and PGC-1α deficient 3T3-L1-derived adipocyte cells, and DRP1 (c), Fis1 (d), OPA1 (e), Mfn2 (f), SIRT1 (g), SIRT3 (h), and COX-I (i) in WT and PGC-1α deficient 3T3-L1-derived adipocyte cells in the absence and presence of EET-A (10 μM) treatment. Results are mean ± SE, = 3-4, versus WT, versus WT, versus WT, and versus WT-EET-A. White bars: WT; black bars: PGC-1α deficient cells.

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