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Journal of Nutrition and Metabolism
Volume 2017, Article ID 2785142, 17 pages
Research Article

Plasma and Aorta Biochemistry and MMPs Activities in Female Rabbit Fed Methionine Enriched Diet and Their Offspring

1Laboratory of Cellular and Molecular Biology, Biochemistry of Extracellular Matrix Remodeling, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, El Alia, 16011 Algiers, Algeria
2Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, Algeria

Correspondence should be addressed to Khira Othmani Mecif; moc.liamy@inamhtok

Received 15 July 2016; Accepted 22 November 2016; Published 4 January 2017

Academic Editor: Michael B. Zemel

Copyright © 2017 Khira Othmani Mecif et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study investigated whether a high Met diet influences biochemical parameters, MMPs activities in plasma, and biochemical and histological remodeling in aorta, in both pregnant female rabbits and their offspring. Four female rabbit groups are constituted (each ), nonpregnant control (NPC), pregnant control (PC) that received normal commercial chow, nonpregnant Met (NPMet), and pregnant Met (PMet) that received the same diet supplemented with 0,35% L-methionine (w/w) for 3 months (500 mg/d). All pregnant females realize 3 successive pregnancies. Plasma results showed that Met excess increased Hcy, raised CRP in NPMet and decreased it in PMet, enhanced significantly proMMP-2 and proMMP-9 activities in NPMet, and reduced them in PMet. Aorta showed a rise in collagen level, essentially in PMet, a reduction of elastin content in both PMet and NPMet, and a significant decrease in lipid content in PMet, with histological changes that are more pronounced in NPMet than PMet. Met excess enhanced proMMP-9 activities in NPMet while it decreased them in PMet. PMet newborn presented increase in uremia and CRP and significant rise of active MMP-2 and MMP-9 forms. In aorta, media and adventitia thickness increased, total lipids content decreased, proMMP-9 activity decreased, and proMMP-2 activity increased.