Diet-Induced Obesity Is Associated with an Impaired NK Cell Function and an Increased Colon Cancer Incidence

Table 1

Characteristics of the specific primers used for real-time RT-PCR analysis.

Gene	Protein	Species	Sequence	bp	NCBI reference
Actb	Beta-actin	Human	Fw: GACGACATGGAGAAAATCTG	131	NM_001101
			Rv: ATGATCTGGGTCATCTTCTC
Ppia	CypA	Rat	Fw: GTGTTCTTCGACATCACG	92	NM_017101
			Rv: TGTCTGCAAACAGCTCGAAG
Klrk1	NKG2D	Rat	Fw: TTAATGAGAACAAAGCCTGG	199	NM_133512
			Rv: GTTAACTCGTTGGGTGATAG
Klrk1	NKG2D	Human	Fw: AGGACAAAATGACCAAAGAC	135	NM_007360
			Rv: CTTGGGGATATCTGAATTGC
Ncr1	NKp46	Rat	Fw: AGACCCTGTTTCTTCTCTTG	197	NM_057199
			Rv: TGAGCTTCTCATGATCCTTC
Ncr1	NKp46	Human	Fv: CAGAAATGTATGACACACCC	119	NM_001145457
			Rv: CTTGAGCAGTAAGAACATGC
Ncr3	NKp30	Rat	Fw: AAGAGCCTCCTCAACAAG	94	NM_181822
			Rv: AGAAAGCTGAGGGCATAG
Ncr3	NKp30	Human	Fw: ATATGCCAAATCTACTCTCTCC	200	NM_001145466
			Rv: TCTGATCCTTCCCATTTCTC