Research Article

Biosynthesis of the Essential Fatty Acid Oxidation Cofactor Carnitine Is Stimulated in Heart and Liver after a Single Bout of Exercise in Mice

Table 1

Real-time polymerase chain reaction primers.

Gene productPrimer sequences

γ-BBHForward primer: AGCAGGACTCCAAGGAGAAT
Reverse primer: CACAAAGGTGGAGGAGAGAA

OCTN2Forward primer: GGGCTACTTCGGACTTTCTC
Reverse primer: GGGAAGAACAAGGTGAGGAT

CPT-1aForward primer: CCAGTCTCAGCCTCTACGGC
Reverse primer: CACAGTGTCCTGTCTCCGTG

18S rRNAForward primer: AGTCCCTGCCCTTTGTACACA
Reverse primer: CGATCCGAGGGCCTCACTA

BBH, γ-butyrobetaine hydroxylase; OCTN, organic cation transporter; CPT-1a, carnitine palmitoyltransferase isoform 1a. Reproduced from Broderick et al. [9] (under the Creative Commons Attribution License/public domain).