Low Citrate Synthase Activity Is Associated with Glucose Intolerance and Lipotoxicity

Figure 7

Palmitate-induced lipotoxicity in C2C12 muscle cells (Cont). C2C12 muscle cells were treated either with control shRNA (Con shRNA) or Cs shRNA which targeted Cs mRNA. Afterwards Con shRNA and Cs shRNA cells were incubated in the media containing 5.5 mM glucose (G) and/or 0.8 mM palmitate (P). (a) Effect of palmitate exposure on the phosphorylation of AMPK (Thr 172) in Con shRNA and Cs shRNA cells using western blot analysis. Quantification of immunoblots from six experiments. P.AMPK was normalized to AMPK. (b) Effect of palmitate exposure on the phosphorylation of PKB (Ser473) in Con shRNA and Cs shRNA cells. Quantification of immunoblots from six experiments. P.PKB was normalized to PKB. Control cells with 5.5 mM glucose were used as reference. Protein load: 25 µg expression. Values are means ± SEM.

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