Research Article

Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes

Figure 3

The effect of lugol on C/EBP-α and PPAR-γ protein expression in mature 3T3-L1 adipocytes. The cells were treated with 0, 1, 10, and 100 µm of lugol for 30 min and 6 h and lysed in RIPA buffer for 1 h. The supernatant was subjected to SDS-PAGE. The blots were blocked and then were incubated with the primary and HRP-conjugated secondary antibodies. Detection was performed according to the enhanced chemiluminescence protocol. (a) 30 min after lugol treatment; (b) 6 h after lugol treatment. All quantitative data are mean ± SD (n = 3/group). and vs. control (vehicle) adipocytes.
(a)
(b)