Table of Contents
Journal of Nanoparticles
Volume 2013 (2013), Article ID 150414, 6 pages
http://dx.doi.org/10.1155/2013/150414
Research Article

Synthesis of Gold Nanoparticles Using Whole Cells of Geotrichum candidum

Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, Punjab 160062, India

Received 31 January 2013; Accepted 12 March 2013

Academic Editor: Hamed Bahmanpour

Copyright © 2013 Amit Kumar Mittal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The synthesis of nanoparticles with desired size and shape is an important area of research in nanotechnology. Use of biological system is an alternative approach to chemical and physical procedures for the synthesis of metal nanoparticles. An efficient environment-friendly approach for the biosynthesis of rapid and stable Gold nanoparticles (AuNPs) using whole cells of Geotrichum candidum is discussed in this paper. The enzymes/proteins present in the microorganism might be responsible for the reduction of metal salts to nanoparticles. Various reaction parameters such as culture age, temperature, pH, metal salt, and cell mass concentrations were optimized. The AuNPs were characterized by UV-visible spectroscopy, dynamic light scattering (DLS), energy dispersive spectroscopy (EDS), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR). Nanoparticles were isolated by sonicating the whole cells after treatment with Tween 80. The whole cell mediated process showed the simplistic, feasible, easy to scale up, and low-cost approach for the synthesis of AuNPs.