Table of Contents
Journal of Nanoparticles
Volume 2014, Article ID 568635, 7 pages
Research Article

The Mode of Action of Silver and Silver Halides Nanoparticles against Saccharomyces cerevisiae Cells

1Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory, building 1-3, Moscow 119991, Russia
2National Research Centre “Kurchatov Institute”, Akademika Kurchatova pl. 1, Moscow 1 123182, Russia
3Institute of Cell Biophysics RAS, Pushchino 142290, Russia
4Skryabin Institute of Biochemistry and Physiology of Microorganisms, Prospect Nauki, Pushchino 142290, Russia
5Vologda State Pedagogical University, S. Orlov Street 6, Vologda 160035, Russia
6Department of Nanoscience and Nanotechnology, Advanced Institute for Science and Technology, Hanoi University of Science and Technology, Dai Co Viet Street, Hai Ba Trung, Hanoi 10000, Vietnam

Received 25 September 2013; Revised 13 November 2013; Accepted 13 November 2013; Published 13 February 2014

Academic Editor: Raphael Schneider

Copyright © 2014 A. A. Kudrinskiy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Silver and silver halides nanoparticles (NPs) (Ag, AgCl, AgBr, and AgI) capped with two different stabilizers (sodium citrate and nonionic surfactant Tween 80) were obtained via sodium borohydride reduction of silver nitrate in an aqueous solution. The effect of the biocidal action of as-prepared synthesized materials against yeast cells Saccharomyces cerevisiae was compared to the effect produced by silver nitrate and studied through the measurement of cell loss and kinetics of K+ efflux from the cells depending on concentration of silver. The results clearly indicate that the silver ions either remained in the dispersion of silver NPs and silver halides NPs after their synthesis or were generated afterwards by dissolving silver and silver halides particles playing a major part in the cytotoxic activity of NPs against yeast cells. It was also supposed that this activity most likely does not relate to the damage of cell membrane.