Table of Contents
Journal of Nanoscience
Volume 2014, Article ID 268293, 7 pages
http://dx.doi.org/10.1155/2014/268293
Research Article

Formulation of a Novel Nanoemulsion System for Enhanced Solubility of a Sparingly Water Soluble Antibiotic, Clarithromycin

1P. D. Patel Institute of Applied Sciences, Charotar University of Science & Technology (CHARUSAT), Changa, Gujarat 388 421, India
2Department of Integrated Biotechnology, Ashok & Rita Patel Institute of Integrated Studies and Research in Biotechnology and Allied Sciences (ARIBAS), Sardar Patel University, Vallabh Vidyanagar, Gujarat 388120, India

Received 21 August 2013; Revised 7 January 2014; Accepted 7 January 2014; Published 23 March 2014

Academic Editor: Sanjeeb K. Sahoo

Copyright © 2014 Stuti Vatsraj et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The sparingly water soluble property of majority of medicinally significant drugs acts as a potential barrier towards its utilization for therapeutic purpose. The present study was thus aimed at development of a novel oil-in-water (o/w) nanoemulsion (NE) system having ability to function as carrier for poorly soluble drugs with clarithromycin as a model antibiotic. The therapeutically effective concentration of clarithromycin, 5 mg/mL, was achieved using polysorbate 80 combined with olive oil as lipophilic counterion. A three-level three-factorial central composite experimental design was utilized to conduct the experiments. The effects of selected variables, polysorbate 80 and olive oil content and concentration of polyvinyl alcohol, were investigated. The particle size of clarithromycin for the optimized formulation was observed to be 30 nm. The morphology of the nanoemulsion was explored using transmission electron microscopy (TEM). The emulsions prepared with the optimized formula demonstrated good physical stability during storage at room temperature. Antibacterial activity was conducted with the optimized nanoemulsion NESH 01 and compared with free clarithromycin. Zone of inhibition was larger for NESH 01 as compared to that with free clarithromycin. This implies that the solubility and hence the bioavailability of clarithromycin has increased in the formulated nanoemulsion system.