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Research Letters in Nanotechnology
Volume 2008, Article ID 789153, 4 pages
Research Letter

Gold and T i O 2 Nanostructurated Surfaces for Assembling of Electrochemical Biosensors

CNR-ISMN, Istituto per lo Studio dei Materiali Nanostrutturati, Via del Castro Laurenziano 19, 7 00161 Roma, Italy

Received 9 December 2007; Accepted 1 February 2008

Academic Editor: Carlos R. Cabrera

Copyright © 2008 Antonella Curulli and Daniela Zane. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Devices based on nanomaterials are emerging as a powerful and general class of ultrasensitive sensors for the direct detection of biological and chemical species. In this work, we report the preparation and the full characterization of nanomaterials such as gold nanowires and T i O 2 nanostructured films to be used for assembling of electrochemical biosensors. Gold nanowires were prepared by electroless deposition within the pores of polycarbonate particle track-etched membranes (PTMs). Glucose oxidase was deposited onto the nanowires using self-assembling monolayer as an anchor layer for the enzyme molecules. Finally, cyclic voltammetry was performed for different enzymes to test the applicability of gold nanowires as biosensors. Considering another interesting nanomaterial, the realization of functionalised T i O 2 thin films on Si substrates for the immobilization of enzymes is reported. Glucose oxidase and horseradish peroxidase immobilized onto T i O 2 -based nanostructured surfaces exhibited a pair of well-defined and quasireversible voltammetric peaks. The electron exchange between the enzyme and the electrodes was greatly enhanced in the T i O 2 nanostructured environment. The electrocatalytic activity of HRP and GOD embedded in T i O 2 electrodes toward H 2 O 2 and glucose, respectively, may have a potential perspective in the fabrication of third-generation biosensors based on direct electrochemistry of enzymes.