Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates
Figure 2
Depiction of the CNT-FET antibody functionalization procedures used in this study. (a) EDC/N-Hydroxy succinimide modification of endogenous CNT carboxyl groups (left). Using this method the antibody is attached to the CNTs via zero-length coupling (right). (b) Pyrenebutanoic acid succinimide esters addition to CNTs (left), which mediate attachment to antibody primary amine groups via a four carbon spacer (right). (c) Addition of pyrene-hydrazide modified antibodies to bare CNT-FETs (left), which can lead to uniform antibody orientation on the circuits (right).