Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates

Figure 4

Comparison of CNT-FET antibody coating procedures. Increasing concentrations of E. coli O157:H7 were added to E. coli O157:H7 polyclonal antibody immobilized onto CNT-FET circuits using either EDC/NHS (□), Pyrenebutyric acid succinimide ester (∘), Pyrene hydrazide modified antibody (▲) or pyrenebutanoic acid/ pyrenebutanoic acid succinimide ester at a molar ratio of 3 : 1 (◊, dotted line) as described in Section 2. Impedance was measured for 4 minutes after addition of the bacteria and plotted as the normalized value compared to the baseline of the individual circuit. Each point represents at least 5 measurements with standard error of the mean.