Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase

Table 1

Enzyme kinetic parameters for the CPO catalyzed peroxidation of pyrogallol.

	(mM)	(mM)	(IU/mg)	(1/sec)
Pyrogallol
Free CPO ( = 0.983)	11 ± 1.0	160 ± 20	2000 ± 80	1400 ± 60
Sol-gel entrapped CPO ( = 0.847)	9.3 ± 2.6	190 ± 74	100 ± 12	70 ± 8.4
H2O2
Free CPO ( = 0.921)	17 ± 6.2	6.4 ± 2.2	9100 ± 2500	6370 ± 1750
Sol-gel entrapped CPO ( = 0.657)	27 ± 39	6.1 ± 8.6	680 ± 830	480 ± 580

Enzyme kinetic parameters were obtained by fitting the data shown in Figure 1(a) (pyrogallol) and Figure 1(b) (H₂O₂) to a substrate inhibition model (see (1)). All parameters are presented as value of the fit ± one standard error of the fit.