Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase

Table 2

Catalytic performance of sol-gel beads loaded with different CPO amounts.

CPO loading of sol-gel beads (µg/mL)	40	8	4
Activity (mIU)	249 ± 33	138 ± 18	76 ± 4
Specific activity (IU/mg)	41 ± 6	115 ± 15	127 ± 6
Relative activity compared to free CPO (%)^#	2.9	8.0	8.80
Cumulative leakage for six days (%)^†	15	12	7

Absolute activity values in mIU (×10⁻³ International Units) and specific activity values per mg initially loaded CPO are presented as mean values ± one standard deviation. All sample sets were prepared in triplicate. ^#The relative activity was based on a reference assay with free CPO in solution. The reference value was 1436 ± 39 IU/mg. ^†The cumulative leakage over six days of maturation corresponds to the summation of the leakage data shown in Figure 2.