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Journal of Nanotechnology
Volume 2016 (2016), Article ID 2547467, 10 pages
http://dx.doi.org/10.1155/2016/2547467
Research Article

Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry

1Department of Human Genetics, Sri Ramachandra University, Chennai, Tamil Nadu 600116, India
2School of Life Sciences, Vels University, Chennai, Tamil Nadu 600117, India
3Department of Biomedical Sciences, Sri Ramachandra University, Chennai, Tamil Nadu 600116, India

Received 6 June 2016; Accepted 17 July 2016

Academic Editor: Paresh Chandra Ray

Copyright © 2016 Venkatachalam Deepa Parvathi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The remarkable advancement of nanotechnology has triggered enormous production of metal nanoparticles and nanomaterials for diverse applications in clinical diagnostics and biomedical research. Nanotechnology has facilitated understanding and analysing nanotoxicology in a holistic approach. Iron nanoparticles have been of special interest in recent research owing to their dynamic, paramagnetic, and catalytic properties. Research studies (in vitro model) have demonstrated the lack of toxicity in nanoiron. The present study design involves in vivo toxicity assessment of nanoiron at specific concentrations of 0.1 mM, 1 mM, 5 mM, and 10 mM in Drosophila. DNA fragmentation assay in exposed and F1 population showed first-line toxicity to flies. Viability and reproductive ability were assessed at 24-hour and 48-hour intervals and thus indicated no statistical significance between the exposed and control groups. The wing spot assay has expressed transparent lack of toxicity in the studied concentrations of nanoiron. Protein profiling has demonstrated that the protein profiles have been intact in the larvae which confirm lack of toxicity of nanoiron. This leads to concluding that nanoiron at the defined concentrations is neither genotoxic nor mutagenic.