Fungus- (<i>Alternaria</i> sp.) Mediated Silver Nanoparticles Synthesis, Characterization, and Screening of Antifungal Activity against Some Phytopathogens

Win, Theint Theint; Khan, Sikandar; Fu, PengCheng

doi:https://doi.org/10.1155/2020/8828878

Journal of Nanotechnology

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2020 | Article ID 8828878 | https://doi.org/10.1155/2020/8828878

Fungus- (Alternaria sp.) Mediated Silver Nanoparticles Synthesis, Characterization, and Screening of Antifungal Activity against Some Phytopathogens

Theint Theint Win,^1,2Sikandar Khan,³and PengCheng Fu¹

Academic Editor: Marco Rossi

Received28 Sept 2020

Revised07 Nov 2020

Accepted16 Nov 2020

Published29 Nov 2020

Abstract

The scientific consensus is now on developing a biocontrol agent that can cause cellular metabolic reprogramming against agricultural pathogens. Biosynthesis of silver nanoparticles was performed by using phytopathogenic fungi (Alternaria sp.) isolated from banana cultivated soil. Alternaria sp. can grow very fast and produce high enough bioactive compounds. This study aims to biosynthesize silver nanoparticles (AgNPs) using fungal Alternaria sp.’s metabolites as a safe antifungal agent against plant pathogenic fungi (Fusarium spp. and Alternaria sp.). To visualize the formation of AgNPs, analytical instruments were used, such as ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, scanning transmission electron microscopy (STEM), energy dispersive X-ray (EDX), and elemental mapping. The UV-visible spectra showed a peak at 435 nm. Analysis of scanning transmission electron microscopy (STEM) micrographs evidenced that the size of synthesized silver nanoparticles ranged between 3 and 10 nm. The resulting AgNPs showed distinct antifungal activity against selected plant pathogenic fungi. Synthesized AgNPs have demonstrated remarkable potential for the use of antifungal compounds to combat plant diseases.

1. Introduction

Biological control agents should be prepared with less risk to human and livestock [1]. Currently, the use of biological control agents at the commercial level has some limitations, such as adaptability to biotic and abiotic factors and deterioration of the desired activity between in vitro and in vivo [2]. It is time to develop more effective and nonpersistent biopesticides with the aid of nanotechnology using biogenic silver, zinc, copper, gold, and iron [3].

Fungitoxic metabolites (antibiotics and other bioactive metabolites) are shown to contribute to biocontrol applications in agriculture [4]. Fungi can produce a considerable amount of extracellular enzymes such as chitinases, glucanases, proteases, glycosyl hydrolases, xylanases, cellulases, and mannanases under suitable conditions [5]. Because of their conformity with the ideals and concepts of green chemistry, metallic nanoparticles from biological sources have received a great deal of attention [6].

Nanosized silver particles have become more popular as technological advancements make their processing more economical [7]. Many attempts have been made to synthesize nano-based antifungal compounds from zinc oxide and silver nitrate used for the effective management of plant pathogenic fungus, including Fusarium oxysporum, Penicillium digitatum, Alternaria citri, Alternaria alternate, and Aspergillus niger [8, 9]. Copper nanoparticles that exhibited strong antifungal activities against tested plant pathogenic fungi responsible for crop diseases were also synthesized by Kanhed et al. (2014) and Bramhanwade et al. (2016) [10, 11]. One of the possible ways in which silver can be used is the control of plant diseases. Since silver displays several modes of inhibitory action on microorganisms [12], it can be used relatively safer than synthetic fungicides [13]. The efficacy and wide range of antimicrobial activity of AgNPs against plant phytopathogenic fungi were also shown by Al-Zubaidi et al. (2019) [14]. However, the restricted study has so far demonstrated its applicability of silver for managing plant diseases [13].

For the control of some plant fungal diseases, biogenic nanoparticles have proved to be effective nanomaterial-based fungicides. Therefore, they possess the potential for extensive use in agriculture as biocontrol agents for promoting sustainable agriculture [3]. This study aimed to synthesize environmentally-friendly biogenic AgNPs by using metabolites from a fast-growing fungus Alternaria sp. for the effective control of some phytopathogenic fungi. Alternaria sp.’s extracts were used for the first time in this study and were found to be highly effective in the reduction of silver ions into silver nanoparticles. Synthesis of metal NPs based on fungal extract is found to be the most effective and environmentally-friendly way of preparing biocontrol agents for phytopathogens suppression.

2. Materials and Methods

2.1. Reagents and Instruments

Silver salts (analytical grade) and culture media were purchased from Aladdin, China, and the solvent used throughout the experiments was double distilled water (ddH₂O) (Millipore 18.2 MX cm). Instruments used in this study were high-speed refrigerated centrifuge (JW-3021HR), UV/Vis spectrophotometer (Shimadzu, UV-3600), scanning transmission electron microscope (STEM), energy-dispersive X-ray (EDX) (JEOL JEM-ARM200F 200 kV), zeta potential (HORIBA Zetasizer SZ100), and FTIR (Nicolet 6700 FTIR spectrometer).

2.2. Fungal Isolation, Characterization, and Preparation of Metabolites

Alternaria sp. was isolated from severely wilt affected banana plants rhizosphere soil in the way below: soil samples were collected from Guilin, China. The serial dilution method was used to isolate fungal pathogens and cultured on PDA (potato dextrose agar) plates, which were incubated at 26–28°C for 5 days. Purified cultures were visually identified using their cultural and microscopic morphology. Presumptive identifications were confirmed with internal transcribed spacer (ITS) rDNA sequence analysis. The entire ITS region of the fungal isolates was amplified with the primer pair ITS1F (5′-CTTGGTCATTTAGAGGAAGTAA-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) [15]. The resulting amplicons were sequenced by a 3730 XL DNA analyser (Applied Biosystems, USA). Sequence identification was performed and deposited to NCBI, BLAST database (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Nucleotides). Its accession number is MN096578.

Alternaria sp. (seven days old) was grown in 200 ml of PDB (potato dextrose broth) at 26–28°C for 5 days. Biomass was recovered by centrifugation at 7000 rpm for 10 min. The supernatant was decanted, and the biomass pellet was subsequently washed with sterilized deionized water to remove all the remaining components of the growth medium. After washing, the biomass was resuspended in 100 ml of sterilized ddH₂O, incubated at 28°C for 3 days, and then filtered through a 0.22 µm membrane filter to get small-molecular-weight metabolites. The resulting filtrates were used for silver nanoparticles synthesis [16].

2.3. Extracellular Biosynthesis of Silver Nanoparticles

For the preparation of AgNPs, 10 ml of the Alternaria sp.’s metabolic filtrate was treated with 50 ml of 1 mM silver nitrate (AgNO₃) solution and kept on stirring (500 rpm) at room temperature (25 ± 1°C) [16]. The development of reddish brown color in the reaction mixture designates the synthesis of AgNPs. Optical property such as localized surface plasmon resonance (LSPR) for the AgNPs was identified by UV-visible spectrometry (Shimadzu UV3600, Japan). The reaction was terminated after SPR bands saturation. The acquired product in suspension was recovered by centrifugation at 12,000 rpm for 15 min. The collected AgNPs were repeatedly washed and freeze-dried. The fungi-mediated green synthesis of silver nanoparticles was further used for characterizing their properties and examining their antifungal activity against phytopathogens [17].

2.4. Characterization of Silver Nanoparticles

2.4.1. UV-Visible Spectroscopy Analysis

AgNPs detection was primarily performed by visual examination of fungal filtrate color change after silver nitrate treatment. The appearance of dark brown fungal cell filtrate suggests AgNPs formation. Besides, the biogenic AgNPs synthesis was further confirmed using UV-Vis absorption spectra. Optical property such as localized surface plasmon resonance (LSPR) for the AgNPs was identified by UV–visible spectrometry (Shimadzu UV3600, Japan). The reaction was terminated after SPR band saturation. The absorption spectra of the samples were measured using a spectrophotometer (Shimadzu UV-3600, Japan) in the wavelength range of 190–800 nm; ddH₂O distilled water was used as blank.

2.4.2. Scanning Transmission Electron Microscope (STEM) and Energy-Dispersive X-Ray (EDX) Analysis

To prepare dried AgNPs powder in the fungal metabolites, treated broth was centrifuged at 12000 rpm for 15 minutes. Supernatants were discarded, and AgNPs pellets were washed three times with autoclaved distilled water and freeze-dried [16]. The dried AgNPs were dispersed and transferred to Cu 200 mesh TEM grids having few nm thick carbon film and dried for 24 h at 60°C. The AgNPs were analyzed using a JEOL JEM-ARM200F 200 kV STEM. The instrument was equipped with a light element 100 mm² SDD EDS detector. Mineralogical information and two-dimensional elemental maps were obtained for Ag, C, and K by electron diffraction of selected areas using a spatial resolution of 50 nm. The particle size was estimated by the SEM result and confirmed by the STEM result.

2.4.3. Zeta Potential

In order to determine the surface electric charge of dried AgNPs as prepared above, zeta potential measurement was carried out by using Zetasizer (Zetasizer SZ-100, HORIBA) [18].

2.4.4. Fourier-Transform Infrared (FTIR)

The freeze-dried powder of AgNPs as prepared above was subjected to FTIR analysis. FTIR spectral bands in the prepared materials (AgNPs) were determined using the FTIR spectrometer (Nicolet 6700, Thermo Scientific), 400–4000 cm⁻¹ in transmittance mode. Samples for FTIR analysis were prepared using the potassium bromide (KBr) pellet technique, which involves mixing the AgNPs thoroughly with KBr before forming a pellet at high pressure.

2.5. Phytopathogens Collection

The plant-pathogenic fungi used in this study were Alternaria sp., Fusarium oxysporum, Fusarium moniliforme, and Fusarium tricinctum. Alternaria sp. was isolated from banana rhizospheric soil. The latter three were provided by Dr. Jing LV, State Key Laboratory of Heavy Oil Processing, University of Petroleum, Beijing, China. All the fungi were grown on potato dextrose agar (PDA) according to the manufacturer’s instructions. Fusarium oxysporum appeared as an abundant cottony white colony with white aerial mycelium. Fusarium moniliforme had a black colony on the backside with a white aerial and marginal mycelium. Fusarium tricinctum colony had abundant cottony white mycelium.

2.6. Screening of Antifungal Potential of Silver Nanoparticles

The well diffusion method was used to assess the antifungal potential of the biosynthesized AgNPs against four pathogenic strains (Fusarium oxysporum, Fusarium moniliforme, Fusarium tricinctum, and Alternaria sp.). Petri dishes were prepared with PDA agar medium having wells of 8 mm diameter at equal distance. After inoculation, the wells were loaded with different concentrations (25, 50, and 100 µl) from the stock solution of AgNPs (1 mg · ml⁻¹). The plates were incubated at 28 ± 1°C, and after 5 days the inhibition zones were examined [19]. Comparison of the means of inhibition of mycelial growth was subjected to one-way ANOVA in order to identify the significance of the activity. The significant differences in each activity were determined by the t-test .

3. Results and Discussion

3.1. Synthesis of Nanoparticles and UV-Vis Spectral Analysis

Alternaria sp. was isolated and grown on plates for extract preparation (Figure 1(a)). The microscopic morphology of the fungal spore is shown in Figure 1(b). Phylogenetic tree of Alternaria sp. and related species based on confidently ITS sequences constructed with neighbor-joining is presented in Figure 1(c). After mixing the Alternaria sp. extracts with the AgNO₃ solution, it was noted visually that the color of the mixture was changed from transparent to dark brownish (Figure 2). The color changed to dark brown as a consequence of the reduction of silver ions with the formation of AgNPs [18]. After 30 min of reaction, both alterations in color and the absorbance were recorded at regular intervals, as the color change was the first evidence of success in nanoparticle formation. Generally, the color change occurred due to reducing agents released from fungal extracts into the solution [16]. This was also supported by UV-visible spectra; when exposed to the light of a specific wavelength, the NPs displayed a unique surface phenomenon called surface plasmon resonance (SPR); due to this, a specific peak formation occurred for each kind of NPs by UV-Vis spectroscopy [20]. The synthesis of AgNPs using (Alternaria sp.) extract was monitored using UV-Vis spectroscopy; the light absorption pattern of the fungal biomass was observed in the range of 350–500 nm.

(a)

(b)

(c)

(a)

(b)

(c)

The silver ions reduction peak of the SPR occurred at 435 nm (Figure 3). The same peak area for AgNPs at 435 nm was also reported by other researchers; one of them used incubation of Ag ions with fungal biomass of Trichoderma koningii [19]. Spectral analysis showed that the UV-Vis SPR peak at 435 nm typically observed for AgNPs that without altering the peak position increased as a function of time. It further confirmed the conversion of silver ions into silver nanoparticles. This observation suggested the Alternaria sp. mediated the biogenic formation of monodispersed AgNPs. It is obvious that SPR peaks of metal nanoparticles depend on particle size, shapes, and reaction medium. The SPR peaks normally show a redshift with an increase in the particle size [21]. In this study, the SPR peak retained its spectral position, confirming a uniform size particle distribution. Fungal extracts are rich in bioactive molecules and functional groups like –OH, amide, and carboxylic groups, which improved the quantitative production of AgNPs and their stable dispersion [22]. Therefore, fungal extracts have the power to be used for the biogenic synthesis of nanoparticles.

We measured UV-Vis spectra of the reacting sample in regular time intervals of 30 min to 24 hr to study the effect of the reaction time. Initially, the color of the filtrate was pale-yellow. As time passes, the color shifts to brown, followed by dark brown with increased absorbency, which suggests the continuous synthesis of AgNPs in filtrate (Figure 3). Moreover, the stability was evaluated after 30 days of synthesis and there was no change in absorption peak value, indicating that the nanoparticles are highly stable after one month under ambient conditions (28°C). Only one plasma band is obtained in the case of monodispersed nanoparticles. The increased intensity indicates a progressive degree of reaction with a corresponding increase in the number of particles [16].

3.2. Characterization of Fungi-Based Synthesis of AgNPs

3.2.1. Zeta Potential Analysis

The zeta potential analysis of the biosynthesized AgNPs was found as a single sharp peak between −60 and 0 mV while having a maximum intensity at −31.9 mV (Figure 4). It indicates that the negatively charged moieties are present on the surface of the AgNPs that expanded in the medium. The repulsion among the particles might be due to the negative values that proved that the particles are very stable. Low zeta potential values of particles suggest no flocculation and no tendency to assemble due to repulsion forces among particles [23, 24].

3.2.2. Scanning Transmission Electron Microscopic (STEM) Analysis

Morphology and size distribution of the biosynthesized AgNPs were revealed via STEM analysis. The prepared AgNPs exhibited a spherical morphology (Figures 5(a) and 5(b)). The biogenic AgNPs displayed a particle size range of 3–10 nm (Figure 5(c)). It is also evident that the biomolecules in the extracts of Alternaria sp. promoted the synthesis of AgNPs. The SEM images of AgNPs reported by other researchers from different extracts showed spherical particles, aggregated spherical particles, irregularly shaped particles, and cubic particles [16, 25]. The moderate particles’ sizes observed were 5, 12, 25, 35, and 50 nm, for AgNPs synthesized by different biological extracts using water as a solvent. Similar results were also found by other researchers recently [16, 25–28]. In an article comparing the advantages and drawbacks of these methods and the applications of nanoparticles in various domains, a synthesis of chemical, physical, and biological methods for obtaining AgNPs of different shapes and dimensions (from 2 to 300 nm) was described [29].

(a)

(b)

(c)

(d)

3.2.3. Energy-Dispersive X-Ray (EDX) Analysis

EDX of the AgNPs was performed to investigate the elemental composition of the biosynthesized AgNPs (Figure 5(d)). The EDX spectra revealed the presence of silver peaks around 3 and 3.1 keV, which appeared due to the discharge of different electrons from L and K shells of silver, respectively. Therefore, the EDX pattern clearly indicates that the AgNPs are crystalline in nature. The lower energy peak (3 keV) was responsible for the outer shell electrons (L), and a higher energy peak (3.1 keV) was responsible for inner shell electrons (K). The observation was in agreement with the previous report by Kasithevar et al. [27]. The carbon peak present in the spectra was mainly due to the carbon adhesive tape used, and the rest of the peaks might be due to inorganic impurities in the biomolecules from the Alternaria sp. extract. The EDX data of AgNPs showed that the weight percentage of Ag was 92%. This is also in agreement with the results reported earlier [28].

3.2.4. FTIR Spectral Analysis

FTIR was employed to quantify and determine the functional biomolecules in the Alternaria sp. extracts, which were important for reducing silver ions into relative silver nanoparticles. The Alternaria sp. extracts have a variety of biomolecules, which might be involved in the synthesis of these AgNPs (Figure 6). The occurrence of many prominent peaks in the IR region of the electromagnetic spectrum is due to different functional groups. The active and extended band was observed at 3421 cm⁻¹ that confirmed the presence of polyphenolic –OH group. The second band was observed relative to the alkyl group of C–H stretching vibrations at the absorption band 2870 cm⁻¹; the third narrowband occurred at 2815 cm⁻¹ is ascribed to C–H of alkane group. The fourth peak at 1638 cm⁻¹ indicating the presence of amide I group; the fifth short band appeared at 1450 cm⁻¹ assigned to carboxylic acid while the sixth peak around 1156 cm⁻¹ is representing stretching of the aromatic ring, and the seventh band at 1027 cm⁻¹ corresponds to C–N stretching vibrations of the aliphatic amines of protein. The last wide and short band at 470 cm⁻¹ represented alkyl halides. This investigation also demonstrated that protein and amino acids are the main components and have the capacity to bind metals. It is evident that polysaccharides, sulfonated compounds, and amide linkages are strongly involved in the reduction of silver ions into nanoparticles. The biomolecules present in fungal extract have dual functions of reducing silver ions and stabilizing the NPs [26, 30].

3.3. Antifungal Potential of the AgNPs

The well diffusion method was used to assess the antifungal potential of the biosynthesized AgNPs against four phytopathogenic strains of fungi (Fusarium oxysporum, F. maniliforme, F. tricinctum, and Alternaria sp.). Recent research of the antifungal activity of AgNPs was obtained by green synthesis mediated by different plants and microbes [19, 31, 32], evaluated primarily by agar well diffusion method. This is consistent with the results of our research and showed comparable values representing antifungal action. Twelve Petri dishes were prepared with PDA medium having wells of 8 mm diameter at equal distance. After inoculation, the wells were loaded with different concentrations (25, 50, and 100 µl) from a stock solution of AgNPs (1 mg · ml⁻¹). The plates were incubated at 28 ± 1°C, and after 5 days, the inhibition zones were measured (Figure 7). Table 1 summarizes the inhibition zone diameter of AgNPs against four phytopathogens.

The minimum inhibitory concentration (MIC) of AgNPs was 25 µl while the growth rates of all strains of fungi were quite low at 50 and 100 µl. The diameter of inhibition zones was measured as a score, indicating that the inhibition zones’ diameter increased with increasing the concentration of AgNPs. The average diameters of inhibition zones were 14.7 ± 1.5 mm, 17 ± 2 mm, and 21.3 ± 1 mm at 25, 50, and 100 µl, respectively, for Fusarium oxysporum (Figure 7(a)). Inhibition zones for Fusarium moniliforme were measured as 9 ± 2 mm, 11.3 ± 1.5 mm, and 17.5 ± 1.3 mm at 25, 50, and 100 µl concentrations of AgNPs, respectively (Figure 7(b)). Inhibition zones for Fusarium tricinctum were 11 ± 2 mm, 11.5 ± 1.3 mm, and 21.2 ± 1 mm at 25, 50, and 100 µl concentrations of AgNPs, respectively (Figure 7(c)). For Alternaria sp., the average diameters of inhibition zones were 17.1 ± 1.02 mm, 19.5 ± 1.5 mm, and 21.6 ± 1.5 mm at 25, 50, and 100 µl, respectively (Figure 7(d)). The maximum inhibition zone was observed at 100 µl concentration of AgNPs for Alternaria sp. (21.6 ± 1.5 mm) following Fusarium oxysporum (21.3 ± 1 mm), suggested the efficacy of the biosynthesized NPs against the phytopathogenic fungal strains. Birla et al. (2013) comparatively studied antimicrobial activity of both chemical AgNO₃ and biogenic AgNP; higher inhibition zone diameter values were observed for the pathogenic fungi by biogenic AgNPs [16]. It was found that the inhibition zone increased as the concentration of AgNPs increased. This may be due to the high solution density, which could saturate and cohesively deactivate the fungal hypha [32]. It has also assumed that Ag⁺ mainly affects membrane-bound enzyme functions, such as those in the respiratory chain, leading to loss of cellular integrity and osmotic balance resulting in acute cell toxicity [33–35]. The observed antifungal activity in this study may be due to the destruction of membrane integrity. According to the previous studies, this is because AgNPs bind and anchor to the cell surface. This interaction causes structural changes and damage that significantly disrupts essential cell functions such as permeability, causes pit and gaps to depress respiratory chain enzyme activity, and eventually leads to cell death [36–38]. In conclusion, AgNPs exhibited strong antifungal effects on tested fungi, possibly by destroying the membrane’s integrity; thus, it was concluded that AgNPs had considerable antifungal activity, which required further investigation for field applications. To conclude, AgNPs have shown strong antifungal effect, possibly by destructing membrane integrity, on fungi tested; thus, AgNPs were assumed to have a significant antifungal activity, requiring further investigation for clinical applications.

4. Conclusions

The green reduction and biogenic synthesis of nanoparticles is an effective, low-cost, sustainable, and environmentally-friendly approach. The phytopathogenic Alternaria sp.’s extract was used for the first time in this study and was found to be highly effective in the reduction of silver ions into silver nanoparticles. Compared with the earlier various biological extracts reported elsewhere, the Alternaria sp. was found to be more effective in silver reduction, as the NPs obtained as a result of treatment with this extract has the lowest zeta potential (−31.9 mV). Furthermore, the fungi-mediated AgNPs were utilized as efficient biocontrol agents for suppressing various fungal phytopathogens. The maximum inhibition zones 21.6 ± 1.5 mm and 21.3 ± 1.0 mm were observed at 100 µl concentration of AgNPs for Fusarium oxysporum and Alternaria sp., respectively. This suggests the efficacy of the biosynthesized NPs against the fungal phytopathogens. It is concluded that fungal extract-based synthesis of metal NPs is the most efficient and environmentally-friendly way to prepare biocontrol agents for phytopathogens suppression. The parameters for synthesis and in vivo antifungal activity should be carefully studied in the future in order to make use of the full potential of AgNPs from Alternaria sp.’s metabolites, which have shown themselves to be a possible antifungal agent to control phytopathogens.

Data Availability

No data were used to support this study.

Conflicts of Interest

The authors declare that they have no conflicts on interest presented in this paper.

Acknowledgments

TTW and PCF are thankful for the financial support by the Research Start-Up Funds from Hainan University in China (KYQD_ZR2017212). SK is grateful for the support by Shaheed Benazir Bhutto University, Pakistan.

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Copyright © 2020 Theint Theint Win et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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