Aspirin Has Antitumor Effects via Expression of Calpain Gene in Cervical Cancer Cells

Figure 3

In vitro characteristics and tumorigenicity of stably transfected cells. (a) Cell proliferation. Control (HeLa), vector-transfected (pCR/HeLa), and mu-type calpain-transfected cells (pCRCAL/HeLa) were counted by the trypan blue staining and Coulter counter. (b) Caspase-3 activities. Caspase-3 activities were measured by analyzing the fluorometric cleavage of substrates, z-DEVD-AFC. RFU, relative fluorescence units. (c) Groups of five mice each were injected with HeLa, pCR/HeLa, and pCRCAL/HeLa cells (1.5×10⁷ in 0.1 mL saline) subcutaneously inducing solid tumor. The tumor volume was evaluated on the 20th, 34th, 41st, and 49th day onwards after tumor cell induction. The tumor volume was calculated by the formula:volume(mm³)=(squarerootofwidth×length)³. Mean values of five mice/group ±SEM are shown. The experiment was repeated three times with similar results. ∗,𝑃<.05 HeLa versus pCRCAL/HeLa.

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