Potential Role of Thymosin-1 Adjuvant Therapy for Glioblastoma
Figure 2
Talpha1 is not directly cytotoxic for (a) rat 9L glioblastoma cells or (b) normal rat cortical neurons, and (c) does not inhibit cell migration. 9L cells and cortical neurons were cultured in 96-well plates and treated with different concentrations of Talpha1. Viability was measured using the Crystal violet assay, and mitochondrial function was measured using the MTT assay. Both the 9L cells and cortical neurons remained viable and exhibited minimal reductions in mitochondrial function over a broad range of Tapha1 exposure. In cultures treated with 50 mM Talpha1 (highest dose used), both cell types exhibited approximately 20% reductions in MTT and CV indices; however, that concentration was considerably higher than the amounts used for the in vivo experiments. (c) In vitro motility assays were performed using 9L cells that were treated with vehicle or Talpha1 (10 mM) for 24 hours. Motility was measured using the ATP Luminescence-Based Motility/Invasion assay (see Methods). The percentages of total migrated, nonmigrated, migrated-adherent (passed through the pores and remained adherent to the undersurface of the membrane), and migrated nonadherent cells (passed through the pores and landed at the bottom of the chamber) were calculated, and the means ± S.D.s of the results are depicted graphically.
