Synergistic Induction of Apoptosis in Primary B-CLL Cells after Treatment with Recombinant Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand and Histone Deacetylase Inhibitors
HDAC inhibition prior to TRAIL administration primes cells for death via alterations in levels of apoptotic regulators. B-CLL cells were cultured with or without 2.5 M Oxam or 500 ng/ml TSA for 16 hours prior to cell harvest and RNA preparation. (a) The fold change in mRNA levels for the indicated pro-apoptotic regulators is shown. (b) The fold change in mRNA levels for the indicated antiapoptotic regulators is shown. One experiment each, representative of more than 5 experiments using cells from different donors, is shown.