AP-2 Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes
Figure 5
Both wild type and dominant negative AP-2 inhibit endogenous c-myc expression. HaCaT cells were infected with Ad-Bgl II (Control), wild type AP-2, dominant negative AP-2, or c-MYC adenovirus at 100 MOI. Twenty-four hours later, nuclear protein and total RNA were extracted. (a) c-MYC mRNA level decreased to about 50% compared to control group after AP-2 infection ( .05 versus BglII control, = 3). (b) c-MYC protein level decreased after AP-2 infection, The fourth lane is a positive control sample from Ad-c-MYC infected HaCaT cells (representative blot of three independent experiments).
