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Journal of Oncology
Volume 2009 (2009), Article ID 963037, 8 pages
http://dx.doi.org/10.1155/2009/963037
Research Article

A Combination of Systemic and Intracranial Anti-CD25 Immunotherapy Elicits a Long-Time Survival in Murine Model of Glioma

Laboratory of Immuno-Oncology and Tumor Immunotherapy, Immunology Program, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, Canada J1H 5N4

Received 8 August 2009; Revised 21 December 2009; Accepted 29 December 2009

Academic Editor: Susan Chang

Copyright © 2009 Marie-Denise Poirier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Abrogating the suppression of glioma-infiltrating Tregs in the periphery and the central nervous system is essential to successful glioma rejection. We sought to improve the immune response in glioma-bearing mice, by investigating new strategies using the anti-CD25 immunotherapy. We found a complete long-term survival of glioma-bearing mice treated with a combination of systemic and intracranial anti-CD25 mAb immunotherapy as compared to systemic administration of anti-CD25 mAb. In addition, the group of mice that had been cured by the combined anti-CD25 mAb showed long-term survival without late tumor relapse when challenged with the GL261 glioma. The antitumor immune response was investigated by analysis of antitumor immune response (CTL). Results showed that the use of the combined injections of anti-CD25 mAb induced efficient targeting of Tregs expansion inside and outside of the brain and altered Tregs trafficking in the bone marrow and brain areas where antitumor immunity was primed.