Journal of Oncology / 2010 / Article / Fig 4

Research Article

Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal

Figure 4

RT-PCR analysis of mRNA expression of laminin ๐›ผ 3, ๐›ฝ 3, and ๐›พ 2 genes in LPA-treated A431 cells. To confirm the results of the previously performed microarrays, RT-PCR analysis was performed as described in Section 2. Briefly, A431 cells were treated with PBS or 2โ€‰ ๐œ‡ M LPA for 12 h, total RNA was extracted for each sample, and cDNA was synthesized using reverse transcriptase. Samples were analyzed on a MiQ machine using FastStart SYBR Green Master Mix. The primer sets used are shown in Table 1. Results showed that LPA treatment increased Ln-332 component, ๐›ผ 3, ๐›ฝ 3, and ๐›พ 2, expression (by ~2โ€“2.5-fold), although results were not determined to be significantly different than PBS control treatments (N = 3; P =โ€‰โ€‰.11, โ€‰.08, and .13, resp.).

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