Use of H19 Gene Regulatory Sequences in DNA-Based Therapy for Pancreatic Cancer

Figure 5

Histopathology analyses and H19 transcript detection in orthotopicaly induced tumors in the pancreas. (a) and (b) Both pictures show poorly differentiated carcinoma. The square in picture (a) delimits the malignant tissue; the circled area in picture (b) corresponds to necrotic interface (X20 original magnification for picture (a), X40 original magnification for picture (b). (c) Interface pancreatic acini (right), neoplasm (left), and desmoplastic reaction (middle) (X10 original magnification). Pictures were taken from paraffin sections and correspond to Giemsa stain. (d)–(f) Total RNA was extracted from frozen pancreatic tumors generated by intracapsular injection of PC.1-0 cells into hamsters pancreas. Lane 1: 100 bp marker, Lane 2: mouse control RNA, Lane 3: RNA extracted from tumor generated in the head of the pancreas, Lanes 4 and 5: RNA extracted from two different tumors generated in the pancreas tail in two different animals. (d) PCR products using primers H19_mouse 454. (e) PCR products using H19-mouse 228 primers. (f) Internal control 𝛽-actin mRNA.