An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines
Figure 3
Course of endogenous SphK-1 and endogenous FGF-2 targeting to the cell periphery over time, observed by immunolocalization experiments. FGF-2 and SphK-1 immunolocalization at 0, 30, and 60 minutes after serum addition (lines (a), (b), (c), resp.) Sections 3 m from surface. FGF-2 was detected using Texas red-conjugated secondary antibodies; SphK-1 was detected using FITC-conjugated antibodies. Arrows indicate granules of protein localization.
