Locking Src/Abl Tyrosine Kinase Activities Regulate Cell Differentiation and Invasion of Human Cervical Cancer Cells Expressing E6/E7 Oncoproteins of High-Risk HPV
Figure 7
Comparison between the influence of SKI-606 and Iressa activity on P-cadherin, fascin, Id-1, IGF-R1, and EGF-R expression as well as β-catenin phosphorylation in HeLa cells. (a) Western blot analysis of P-cadherin, fascin, Id-1, IGF-R1, and EGF-R expression in HeLa-untreated and treated cells. We found that SKI-606 decreases the expression of P-cadherin, fascin, Id-1 and IGF-R1 in HeLa cells in comparison with Iressa-treated (as indicated in the Materials and Methods) and untreated cells; in contrast, Iressa largely reduces the expression of EGF-R in comparison with SKI-606. (b) Tyrosine phosphorylation analysis of -catenin in HeLa cells and SKI-606 as well as Iressa-treated cells. Src/Abl inhibitor (SKI-606) blocks the constitutive phosphorylation of Src and consequently -catenin phosphorylation in HeLa cancer cells. Cells were grown for two days in both the absence (control) and presence of 2 and 5 M of SKI-606 and 5 M of Iressa. Cell lysates were immunoprecipitated with anti--catenin antibody and analyzed by immunoblotting with antiphosphotyrosine antibody as described in Section 2.
