Effects of HIF-1𝛼 and HIF2𝛼 on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts

Figure 1

Features of CCRCC 786-0 tumors. (a–d) (x100 hpf) HIF-1$\alpha$ nuclear staining only $({}^{*}P<.0001$). (e–h) Differential HIF-2$\alpha$ nuclear staining $({}^{*}P=.0004$). (i) Clear-cell/sarcomatoid morphology (x40 hpf). (j) CD10⁺ staining typical of clear-cell RCC lineage (x100 hpf). (k) PTEN staining; positive staining only identified in murine fibroblasts (x100 hpf). (l) Akt staining, (m) Phospho-Ser₄₇₃ Akt staining (highest in HIF-2+ tumors). ${}_{}{}^{*}P$ values were calculated using an ANOVA test.

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(i) H+E

(j) CD10

(k) PTEN

(l) AKT

(m) Phospho-${\text{Ser}}_{473}$ AKT